Physical properties of plasmid Mor174, which determines bacteriocin production in Proteus morganii 174.
نویسندگان
چکیده
Plasmid Mor174 has a molecular weight of 3.6 X 10(6) and a buoyant density of 1.6994 g/cm3. The covalently closed circular form has a sedimentation coefficient of 22S. These are 30 to 40 plasmid copies per genome equivalent, but growth in chloramphenicol results in amplification of the copy number to 600. In Proteus morganii 174, Mor174 coexists with a cryptic plasmid of molecular weight 15.8 X 10(6) and a buoyant density of 1.7170 g/cm3.
منابع مشابه
Mobilization of morganocin 174 plasmid and kinetics of morganocin production in Proteus and Escherichia coli hosts.
Morganocin 174 is coded by a plasmid, Mor174. The plasmid is not self-transmissible but may be mobilized by resistance factor R772. Morganocin synthesis in all Proteus and Providencia strains carrying Mor174 was characterized by a longer lag period after induction and higher titers than in Escherichia coli B(Mor174). The low titers obtained in E. coli B(Mor174) are due to a heatstable inhibitor...
متن کاملEffect of Physical and Chemical Changes on the Antimicrobial Activity of Culture Supernatant Fluid of Lactic Acid Bacteria
ABSTRACT Background and Objective: Lactic acid bacteria are Gram-positive, catalase-negative, nonsporulating, either rod- or coccus-shaped bacteria that have beneficial effects on their hosts by producing antimicrobial substances such as lactic acid, hydrogen peroxide, bacteriocins and biosurfactants. Bacteriocins are antimicrobial peptides that a...
متن کاملPlasmid Pattern of Biofilm Producing Proteus mirabilis and Proteus vulgaris among Clinical Isolates in Kerman University Hospitals during 2011-2012
Background & Aims: Emergence of biofilm producing Proteus strains created a serious problem in the treatment of catheter-associated urinary tract infections. The aim of this research was to study biofilm production and plasmid pattern of proteus strains associated with Urinary tract infection. Methods: A total of 88 strains of Proteus were isolated from samples collected in hospitals of Kerman/...
متن کاملThe secreted hemolysins of Proteus mirabilis, Proteus vulgaris, and Morganella morganii are genetically related to each other and to the alpha-hemolysin of Escherichia coli.
Secreted hemolysins were extremely common among clinical isolates of Proteus mirabilis, Proteus vulgaris, and Morganella morganii, and hemolytic activity was either cell associated or cell free. Southern hybridization of total DNA from hemolytic isolates to cloned regions of the Escherichia coli alpha-hemolysin (hly) determinant showed clear but incomplete homology between genes encoding produc...
متن کاملIn vitro-constructed RP4-prime plasmids mediate orientated mobilization of the Proteus morganii chromosome.
RP4-prime plasmids containing inserts of Proteus morganii strain 2815 chromosomal DNA (0.5-13 megadaltons in size) were constructed in vitro. When introduced into the latter organism, the hybrid plasmids promoted transfer of chromosomal markers in an orientated manner, with recombinant frequencies from 10(-4) to 10(-6) per donor. Transconjugants expressed the tetracycline-resistant phenotype of...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید
ثبت ناماگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید
ورودعنوان ژورنال:
- Journal of bacteriology
دوره 133 1 شماره
صفحات -
تاریخ انتشار 1978